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OCR: Cutting DNA Molecules with Restriction Enzymes E.coli DNA Human DNA GAATTC 3 5 GAATTC 3 CTTAAG 3 CTTAAG + 5 | Cut with a restriction # enzyme ( e.g ., Eco RI). | Cut with a restriction # enzyme (e.g ., Eco RI). 5' 3' 5 3 G 3 5' AATTC 5 3 AATTC CTTAA G CTTAA + 3' 3 5 5 ₲ 5 + Complementary ["sticky"] ends Complementary("sticky" ]ends Mix DNA fragments with the same sticky ends + Anneal and join with ligase. Recombinant DNA molecule 5' GAATTC 3 CTTAAG 5